Antioxidant Activity Test Combination Of Cantigi Leaf Extract (Vaccinium varingiaefolium) And Nail Henna Leaf Extract (Lawsonia inermis Linn)

Abstract

Traditionally, cantigi leaves ( Vaccinium varingiaefolium ) are used to heal wounds,
swelling, burning, pain, ulcers, and function as analgesic, anti-inflammatory and antiinflammatory. inflammation, and treat fine wrinkles . henna leaves ( Lawsonia inermis
Linn ) which contains glycosides, steroids, phytosterols, saponins, tannins, and
flavonoids which are reported to have benefits for treating boils, scabies, headaches,
back pain, and can accelerate hair growth . Cantigi leaves and fruit are reported to
contain anthocyanin compounds that function as antioxidants . while the antioxidants
contained in cantigi leaves are Beta.-mono-olein , Hexadecanoic acid, and methyl ester
. The flavonoids contained in henna leaves also have the potential as antioxidants. We
are often exposed to oxidation both produced from within the body or from outside such
as pollution, cigarette smoke, UV radiation and food. Antioxidants will neutralize these
free radicals so they do not have the ability to steal electrons from cells and DNA.
Cantigi leaves and henna leaves were macerated by kinetic maceration method with
70% ethanol as solvent. Antioxidants were measured using the DPPH method, the
single extract of cantigi gave an IC 50 value of 119.23 ± 4.41 µg/mL , while the extract
of a single nail henna gave an IC 50 value of 38.38 ± µg/mL. The combination of
cantigi leaf extract and nail henna leaf extract with a ratio of 1:1; 2:1 and 1:2 gave
very strong IC50 values, namely 33.84 µg/mL; 40.40 µg/mL and 29.85 µg/mL,
respectively. It was concluded that the combination of cantigi leaf extract and nail
henna leaf extract with a ratio of 1:2 gave the best IC50 value of 29.85 µg/mL.