Dengue Virus Detection Using Rt-Pcr Method In Ternate City, North Maluku, Indonesia
DOI:
https://doi.org/10.51601/ijhp.v2i4.98Keywords:
Detection, Dengue Virus, RT-PCR, Ternate CityAbstract
The Dengue disease is caused by a virus from the family Flaviviridae and there are four distinct, but closely related,
virus serotypes that cause dengue (DENV-1, DENV-2, DENV-3 and DENV-4). Dengue virus detection is very
important to determine the virus serotype that develops in an area. There are several methods to detect dengue virus
based on different targets, namely nucleic acids, viral antigens, and antibodies. This study aims to determine the type
of dengue virus serotype in four working areas of the Public Health Center in Ternate City. The sample in this study
was the eggs of Aedes sp mosquitoes caught in the homes of DHF sufferers and the houses around them. Collection of
mosquito eggs Ae. Aegypti was carried out in four Puskesmas working areas in Ternate City. Ovitrap installation was
carried out in 200 houses, with a total of 400 ovitraps. Rearing eggs and dengue virus detection were carried out at
the Microbiology Laboratory, LITBAGKES Banjarnegara. The method used in this research is the RT-PCR test. The
data analysis of the research results was carried out descriptively. Based on the identification results, the mosquito
used in this study was the Aede aegypti mosquito. The results of electrophoresis produced viral RNA with a base
length of 100 bp, while the target RNA that had been determined were Den1 = 342 bp, Den2 = 251 bp, Den3 = 538
bp. Den4 = 752 bp. The results of the RT-PCR examination showed that the Ae. aegypti in the four working areas of
the Puskesmas did not contain the dengue virus (virus negative).
References
. Basurko C , Matheus S ,3 Hilderal H, Everhard S, Restrepo M, Cuadro-Alvarez, Lambert V, Boukhari R, Duvernois Jean-Pierre, Favre A, Nacher M, and Carles G. Estimating the Risk of Vertical Transmission of Dengue: A Prospective Study. Amrecian Journal Of Tropocal Medicine and Hygiene. 2018. 00(0), pp. 1–7
. The Ministry of Health of the Republic of Indonesia. The Situation of Dengue Fever Cases in Indonesia in 2016 [Infodatin] Data and Information Center of the Ministry of Health RI. 2017.
. World Health Organization (WHO). Dengue and severe dengue. 2017 (Accessed January 9, 2022). ttps://apps.who.int/ mediacentre/factsheets/fs117/en/index.html
. Ministry of Health of the Republic of Indonesia (ID). Guidelines for the Control of Dengue Hemorrhagic Fever in Indonesia. Indonesian Ministry of Health. Director General of P3L. 2015. Jakarta
Hikmawati I, Huda S. The Role of Mosquitoes as Vectors of Dengue Hemorrhagic Fever (DHF) Through Transovarial. Publisher and Printer: Satria Publisher. 2021. p.125
. Amarullah IH, Sucipto TH, Churrotin S. Handbook of Dengue Virus Isolation and Detection. Institute of Tropical Diseases, Airlangga University. Campus C Jl Mulyorejo Surabaya 60115. 2021. p.60
. Grunnill M, B. M. How Important is Vertical Transmission of Dengue Viruses by Mosquitoes (Diptera: Culicidae)?', Jurnal Medical Entomology. 2016. 53(1):1–19.
. World Health Organization (WHO). Dengue and severe dengue. 2021. (Accessed January 9, 2022). https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue
. Wowor M F. Early Detection of Dengue Hemorrhagic Fever With Ns1 . Antigen Examination Early Detection of Dengue Hemorrhagic Fever With Ns1 . Antigen Examination. Jurnal Biomedik. 2011. 3(1).1-9
. Sorisi A.M.H. Transmisi Transovarial Virus Dengue Pada Nyamuk Aedes spp. Jurnal Biomedik (JBM). 2013. 5(1):26-31
. Putri DF , Widiani N , Arivo D. Transovarial Spread of Dengue Virus on Dengue Hemorrhagic Fever Vector Aedes aegypti Mosquito. Holistic Health Journal Transovarial Spread of Dengue Virus on Dengue Hemorrhagic Fever Vector Aedes aegypti Mosquito. Holistic Health Journal. 2018. 12(4): 216-223
. Ternate City Health Department, 2021. Ternate City Health Profile. Year 2021.
. Sasmono T, Yohan B, Setainingsih TY, Aryati, Wardhani P, Rantam FA. Identifikasi Genotipe dan Karakterisasi Genome Virus Dengue di Indonesia untuk Penentuan Prototipe Virus Bahan Pembuatan Vaksin Dengue Berbasis Strain Indonesia. Prosiding seminar Insentif Riset SINas. 2012. (InSINas 2012)
. Fadila Z, Hadi UK, Setiyaningsih S. Bioecology of dengue hemorrhagic fever (DHF) vectors and detection of dengue virus in Aedes aegypti (Linnaeus) and Ae. albopictus (Skuse) (Diptera: Culicidae) in the dengue endemic village of Bantarjati, Bogor City. Indonesian Journal of Entomology. 2015. 12(1):31–38
. Wijayanti SPM, Porphyre T, Chase-Topping M, Rainey SM, McFarlane M , Schnettler E , Biek R, Kohl A. The Importance of Socio-Economic Versus Environmental Risk Factors for Reported Dengue Cases in Java, Indonesia. PLOS Neglected Tropical Diseases. 2016. 10(9): 1-15
]. Wesolowskia A, Qureshic T, Boni MF. Sundsoy PR , Michael A. Johansson MA, Rasheed SB , Engø-Monsen K , and Buckeea CO. Impact of human mobility on the emergence of dengue epidemics in Pakistan. Proceedings of the National Academy of Sciences of the United States of America. 2015. 112(38): 11887-92
. Sirisena P, Noordeen F, Kurukulasuriya H, Romesh TA, Fernando L. Effect of Climatic Factors and Population Density on the Distribution of Dengue in Sri Lanka: A GIS Based Evaluation for Prediction of Outbreaks. PLoS ONE. 2017. 12(1): e0166806.
. Tomia A. The Relationship of Knowledge, Attitudes and Community Practices to Efforts to Control Dengue Vectors in Ternate City, North Maluku Province. Journal of Health Ecology. 2020. 19(3): 211 - 220
. Sumantri A. Environmental Health. [3rd Edition]. Publisher. date. Prenada Media Grup. Jakarta. 2015. p.332
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Amalan Tomia, Rosmila Tuhatea
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
