Activity Test of Squid Ink (Loligo Sp.) As Antibacterial Against Enterococcus Faecalis and Pseudomonas Aeruginosa Bacteria

Authors

  • Valencia Angelina Halim Medical Professional Education, Faculty of Medicine, Dentistry and Health Sciences, Prima University of Indonesia, Medan, Indonesia
  • Sara Rai Faomasi Duha Medical Professional Education, Faculty of Medicine, Dentistry and Health Sciences, Prima University of Indonesia, Medan, Indonesia
  • OK Yulizal Department of Internal Medicine, Faculty of Medicine, Dentistry and Health Sciences, Prima University of Indonesia, Medan, Indonesia
  • Irza Haicha Pratama Department of Public Health, Faculty of Medicine, Dentistry and Health Sciences, Prima University of Indonesia, Medan, Indonesia

DOI:

https://doi.org/10.51601/ijhp.v3i3.168

Keywords:

Squid ink extract (Loligo sp.), Enterococcus Faecalis, Pseudomonas Aeruginosa, Antibacterial and Bacteria.

Abstract

Squid (Loligo sp.) is an invertebrate animal, part of the cephalopod class. The
squid has a distinctive black ink fluid for self-defense. Squid ink contains melanin
compounds that can be used as an antibacterial. Enterococcus faecalis is a
bacterium that causes endodontic infection. While Pseudomonas aeruginosa is a
bacterium that causes infection in patients with compromised immune system, and
also who using a urinary catheter or intravenous catheter. This study aims to
determine whether squid ink can be used as an antibacterial that can suppress the
growth of Enterococcus faecalis and Pseudomonas aeruginosa bacteria. This
research was conducted with experimental method, true experimental Post-Test
Only Control Group research design. The research by using squid ink was
carried out by inserting extract with various concentration (20%, 40%, 60%, 80%
and 100%) into a petri dish which containing bacterial culture. The final showed
that between concentrations of 20%, 40%, 60%, 80% and 100% were no clear
zones, which had been repeated 3 times against Enterococcus faecalis and
Pseudomonas aeruginosa bacteria. From the results obtained, it can be
concluded that there is no bacterial inhibition caused by squid ink against
Enterococcus faecalis and Pseudomonas aeruginosa.

References

Armianty & K. Indra. (2021). Efektivitas Antibakteri Ekstrak Daun Sirih (Piper Betle Linn) Terhadap Bakteri

Enterococcus Faecalis. Dentofasial, Vol.13, No.1, Februari 2014:17-21. from

https://jdmfs.org/index.php/jdmfs/article/viewFile/381/381.

Jawetz, Melnick, and Adelberg’s (2019). Medical Microbiology (28th Edition). McGraw Hill. (August 30,

: 253-259.

Soedarto, S. (2016). Infeksi Nosokomial Di Rumahsakit: Hospital Nosocomial Infections, Jakarta: Sagung Seto,

: 234.

Rasfayanah, Arni Isnaini Arfah, & Zulfahmidah. (2021). PKM Sosialisasi Penggunaan Antibiotik Dan Efek

Penyalahgunaan Antibiotik Guna Pengendalian Resistensi Antibiotik di Desa Sanrobone Kecamatan Sanrobone

Kabupaten Takalar. Jurnal Pengabdian Kedokteran Indonesia, 2(1), 33–

https://doi.org/10.33096/jpki.v2i1.130

Hossain, M. P., Rabeta, M. S., & Husnul Azan, T. (2019). Medicinal And Therapeutic Properties of Cephalopod

Ink: A Short Review. Food Research, 3(3), 188–198. https://doi.org/10.26656/fr.2017.3(3).

Nasution, F. M., Mardia, R. S., Azri, A., Hutabarat, R. R., Al Izza, F., & Asfur, R. (2017). Pengaruh Pemberian

Ekstrak Tinta Cumi (Squid Ink) Terhadap Aterosklerosis. Jurnal e-Biomedik (eBm) (Vol. 5, Issue 2).

https://doi.org/10.35790/ebm.v5i2.16612

Rocky J. Mangindaan, Christy N. Mintjelungan & Damajanty H. C. Pangemanan (2019). Uji Daya Hambat

Ekstrak Tinta Cumi-Cumi (Loligo Sp) Terhadap Pertumbuhan Bakteri Streptococcus Mutans. Jurnal eBiomedik (eBm), 7(2): 82-86. https://doi.org/10.35790/ebm.v7i2.23877

Apriyanto. R (2017). Pengaruh Pemberian Ekstrak Tinta Cumi-Cumi (Loligo Sp.) Terhadap Biofilm Coverage

Rate Dan Visual 3 Dimensi Biofilm Bakteri Pseudomonas Fluorescens Secara In Vitro. Thesis. Universitas

Brawijaya. 25 p. http://repository.ub.ac.id/id/eprint/7145

Rahayu, M. P., Pangemanan, D. H. C., & Mintjelungan, C. N. (2019). Uji Daya Hambat Ekstrak Tinta CumiCumi (Loligo Sp) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal E-Biomedik, 7(2), 76–81.

https://doi.org/10.35790/ebm.v7i2.23876.

Fitrial, Y., Khotimah, I. K., & L. (2017). Aktivitas Antibakteri Dari Melanin Tinta Sotong Dan Cumi-Cumi.

JurnalPengolahanHasilPerikananIndonesia,IPB.20,266–274.http://journal.ipb.ac.id/index.php/jphpi/article/v.

M.Y., F. Z., M.S., R., Smiline Girija, A. S., Suba, K. P., Hariprasad, G., Raghuraman, R., Hermelin, J., Diaz, J.,

& Thilaga, R. D. (2014). A Novel Study on The Antibacterial Effect of The Crude Squid Ink Extracts from The

Indian Squid Against Four Bacterial Pathogens Isolated from Carious Dentine. International Journal of

Current Microbiology and Applied Sciences. 2014 Vol.3 No.4 pp.904-911 ref.32. https://www.ijcmas.com/vol3-4/A.S.Smiline%20Girija,%20et%20al.pdf

Kurniawan I. (2018) Uji Efektivitas Tinta Cumi-Cumi (Loligo Sp) Terhadap Pertumbuhan Bakteri Salmonella

Thypi. Thesis. Surabaya: Universitas of Nahdatul Ulama. http://repository.unusa.ac.id/id/eprint/3470

Andrea V. Colombo, Graziela M. Barbosa, Daniela Higashi, Giorgio di Micheli, Paulo H. Rodrigues & Maria

Regina L. Simionato (2013). Quantitative Detection of Staphylococcus Aureus, Enterococcus Faecalis and

International Journal of Health and Pharmaceutical

https://ijhp.net

Pseudomonas Aeruginosa in Human Oral Epithelial Cells from Subjects with Periodontitis and Periodontal

Health. Volume 62, Issue 10. Published: 01 October 2013 https://doi.org/10.1099/jmm.0.055830-0

Senji Sakanaka, Mujo Kim, Makoto Taniguchi & Takehiko Yamamoto (1989). Antibacterial Substances in

Japanese Green Tea Extract Against Streptococcus Mutans, A Cariogenic Bacterium. Agricultural and

Biological Chemistry, Volume 53, Issue 9, 1 September 1989, Pages 2307–2311,

https://doi.org/10.1080/00021369.1989.10869665

Yumei Niu, Kun Wang, Sainan Zheng, Yufei Wang, Qian Ren, Haoran Li, Longjiang Ding, Wei Li, & Linglin

Zhang (2020). Antibacterial Effect of Caffeic Acid Phenethyl Ester on Cariogenic Bacteria and Streptococcus

Mutans Biofilms. ASM Journals. Antimicrobial Agents and Chemotherapy. Vol. 64, No. 9.

https://doi.org/10.1128/AAC.00251-20.

Downloads

Published

2022-11-22

How to Cite

Angelina Halim, V. ., Rai Faomasi Duha, S. ., Yulizal, O. ., & Haicha Pratama, . I. . (2022). Activity Test of Squid Ink (Loligo Sp.) As Antibacterial Against Enterococcus Faecalis and Pseudomonas Aeruginosa Bacteria. International Journal of Health and Pharmaceutical (IJHP), 3(3), 311–317. https://doi.org/10.51601/ijhp.v3i3.168

Issue

Section

Articles