Article Review: Phenotypic and Genotypic Identification of Bacillus cereus in Soy Milk
DOI:
https://doi.org/10.51601/ijhp.v6i2.633Abstract
Bacillus cereus is a Gram-positive, spore-forming bacterium frequently found as a contaminant in various food products, including soy milk, and is recognized as a causative agent of foodborne illnesses. Its pathogenicity mainly arises from the production of toxins such as non-hemolityc enterotoxin (nhe), hemolysin BL (hbl), and cytotoxin K (cytK), which contribute to gastrointestinal disorders. Conventional phenotypic identification methods, including culture, morphological observation, and biochemical assays, are simple but often lack specificity in distinguishing B. cereus from the B. cereus group. Therefore, genotypic approaches based on molecular techniques such as PCR and Whole Genome Sequencing (WGS) are required to achieve higher sensitivity and precision. The gyrB gene serves as a molecular marker for species confirmation, while the nhe gene complex acts as a virulence determinant in identifying toxigenic strains. The integration of phenotypic and genotypic methods provides a more comprehensive understanding of B. cereus contamination in soymilk, elucidating its genetic diversity and pathogenic potential, which are essential aspects of food safety assessment.
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[1]. Ahaotu, I., Anyogu, A., Njoku, O. H., Odu, N. N., Sutherland, J. P., & Ouoba, L. I. I. (2013). Molecular identi-fication and safety of Bacillus species involved in the fermentation of African oil beans (Pentaclethra macro-phylla Benth) for production of Ugba. International Journal of Food Microbiology, 162(1), 95–104. https://doi.org/10.1016/j.ijfoodmicro.2013.01.001
[2]. Arisanti, R., Arisanti, R. R., Indriani, C., & Wilopo, S. A. (2018). Kontribusi agen dan faktor penyebab kejadian luar biasa keracunan pangan di Indonesia: kajian sistematis [Contributions of agents and factors causing foodborne disease outbreaks in Indonesia: A systematic review]. Berita Kedokteran Masyarakat, 34(3), 99–106. https://journal.ugm.ac.id/bkm/article/view/33852
[3]. Azizah, S. N., & Octavia, B. (2023). Deteksi cemaran Bacillus cereus, serta analisis kualitas fisik dan kimia susu kedelai (Studi higiene dan sanitasi produksi susu kedelai skala rumah tangga di Sleman, DIY) [Detection of Bacillus cereus contamination and analysis of physical and chemical quality of soy milk (Study on hygiene and sanitation of household scale soymilk production in Sleman, DIY)]. Kingdom: The Journal of Biological Studies, 8(2), 119–130.
[4]. Butovskaya, E., Caprai, E., Peloso, M., Gasparini, M., Borgia, M., Abdul, M. E., et al. (2025). Plant based milk alternatives: Assessing the occurrence of chemical and microbiological contaminants in soy, oat, rice and al-mond beverages from Italian market. Food Control, 169, 111005. https://doi.org/10.1016/j.foodcont.2024.111005
[5]. Cahyani, N. P. M. S. (2019). Perbedaan kualitas bakteriologis susu kedelai produksi home industry berdasarkan variasi suhu penyimpanan [Difference in bacteriological quality of household industry soy milk with different storage temperatures]. Poltekkes Denpasar Repository, 5(1), 1–11.
[6]. Dietrich, R., Jessberger, N., Ehling Schulz, M., Märtlbauer, E., & Granum, P. E. (2021). The food poisoning toxins of Bacillus cereus. Toxins, 13(2), 1–21. https://doi.org/10.3390/toxins13020001
[7]. Dos Anjos, T. R., Cavicchioli, V. Q., Lima, J. A. S., Vasconcellos, A. N., Vaz, A. C. N., Rossi, G. A. M., et al. (2020). Unsatisfactory microbiological aspects of UHT goat milk, soymilk and dairy beverage of goat milk and soy protein: A public health issue. Food Science and Technology, 40(6), 349–354. https://doi.org/10.1590/fst.34919
[8]. Enosi Tuipulotu, D., Mathur, A., Ngo, C., & Man, S. M. (2021). Bacillus cereus: Epidemiology, virulence fac-tors, and host–pathogen interactions. Trends in Microbiology, 29(5), 458–471. https://doi.org/10.1016/j.tim.2020.09.003
[9]. Guo, H., Yu, P., Yu, S., Wang, J., Zhang, J., Zhang, Y., et al. (2021). Incidence, toxin gene profiling, antimicro-bial susceptibility, and genetic diversity of Bacillus cereus isolated from quick frozen food in China. LWT – Food Science and Technology, 140, 110824. https://doi.org/10.1016/j.lwt.2020.110824
[10]. Hasanah, L. N., Muhlishoh, A., Maret, U. S., Rosnah, R., & Kendari, P. K. (2022). Keamanan dan ketahanan pangan [Food safety and food security]. https://www.researchgate.net/publication/366684879
[11]. Hendriani, R., & Budiarso, L. (2020). Identifikasi Escherichia coli pada susu kedelai tak bermerek di kota Tan-gerang [Identification of Escherichia coli in unbranded soy milk in the city of Tangerang]. Tarumanagara Medical Journal, 2(1), 137–140.
[12]. Mustika, S., Insan, R. R., & Faridah, A. (2019). Analisis cemaran Escherichia coli, Salmonella sp., Staphylococcus aureus, dan Bacillus cereus pada minuman susu kedelai di Kota Padang [Contamination analysis of Escherichia coli, Salmonella sp., Staphylococcus aureus, and Bacillus cereus in soymilk drinks in Padang City]. Jurnal Pendidikan dan Kesehatan, 11(2), 1.
[13]. Osama, R., Ahmed, M., Abdulmawjood, A., & Al Ashmawy, M. (2020). Prevalence and antimicrobial re-sistance of Bacillus cereus in milk and dairy products. Mansoura Veterinary Medical Journal, 21(2), 11–18.
[14]. Qu, Y., Wei, C., Dai, X., Bai, Y., Zhao, X., Lan, Q., et al. (2021). The possible transmission and potential en-terotoxicity of Bacillus cereus on lettuce farms in five Chinese provinces. Frontiers in Microbiology, 12, 1–10. https://doi.org/10.3389/fmicb.2021.701827
[15]. Ramarao, N., Tran, S. L., Marin, M., & Vidic, J. (2020). Advanced methods for detection of Bacillus cereus and its pathogenic factors. Sensors, 20(9), 1–21. https://doi.org/10.3390/s20092565
[16]. Storz, M. A., Brommer, M., Lombardo, M., & Rizzo, G. (2024). Soy milk consumption in the United States of America: An NHANES data report. Nutrition Reports, 1–13.
[17]. Widodo, W., Purlinda, D. E., & Riadi, A. (2022). Perbandingan berbagai metode pengecatan spora pa-da Bacillus cereus [Comparison of various spore staining methods on Bacillus cereus]. Jurnal Laboratorium Medis, 4(2), 91–94.
[18]. Yennie, Y., Dewanti Hariyadi, R., Kusumaningrum, H. D., & Poernomo, A. (2022). Contamination of Staphylococcus aureus and Bacillus cereus in sushi at retail level in Jabodetabek area. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(2), 331–344.
[19]. Yu, S., Yu, P., Wang, J., Li, C., Guo, H., Liu, C., et al. (2020). A study on prevalence and characterization of Bacillus cereus in ready to eat foods in China. Frontiers in Microbiology, 10, 1–11. https://doi.org/10.3389/fmicb.2020.02907.
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